Echocardiography measures cardiac function, which is severely compromised in muscular dystrophy murine models. Mice are anaesthetized and placed on a heated image platform, where both heart rate and temperature are monitored to minimize physiological variation. Mice are imaged using a high-frequency echocardiography machine following a standardized protocol to systematically evaluate cardiac size and function. Primary echocardiography measurements are fraction shortening, ejection fractioning and heart rate. We can also measure pulmonary/aortic velocity, left and right ventricle E/A ratio, myocardial performance index, left and right ventricle stroke volume and cardiac output.