Degeneration: lmmunoglobin M (IgM)

Figure 1: IgM staining on diaphragm section of a BL10 mouse. Insert showing lack of degenerating fibers.

Figure 2: IgM staining on diaphragm section of MDX mouse. Insert showing brown stained degenerating fibers.

Figure 3: Degenerating fibers in diaphragm section of BL10 (n=8) versus MDX (n=8) male mice at 14 weeks. Mean with standard error showing statistical significance (***) with a p-value of 0.0002.



IgM staining is used to stain the Immunglobulin M antibody. A DAB counterstain is used to mark IgM stained fields a dark brown under bright field. IgM stained muscle sections can be assessed for degenerating muscle fibers, where the IgM antibody has infiltrated damaged muscle fibers.