TRE-H2Kb x CKM-tTA (HT mice)

Information

Crossbreeding TRE-H2Kb and CRE-tTA produces the mouse model of myositis (the HT mouse model), which can result in muscle weakness in the mice. The onset of disease can be controlled by administering doxycycline (0.2g/L) in the drinking water of the animals. Muscle weakness is readily apparent in female mice by 3 months of age, while males show fewer signs of disease onset. Signs of the disease onset include muscle weakness and reduced locomotor activity.

Related information

Nagaraju, K., Raben, N., Loeffler, L., Parker, T., Rochon, P. J., Lee, E., … Plotz, P. (2000). Conditional up-regulation of MHC class I in skeletal muscle leads to self-sustaining autoimmune myositis and myositis-specific autoantibodies. Proceedings of the National Academy of Sciences of the United States of America97(16), 9209–9214. Publication

Coley, W., Rayavarapu, S., Pandey, G. S., Sabina, R. L., vander Meulen, J. H., Ampong, B., … Nagaraju, K. (2012). The molecular basis of skeletal muscle weakness in a mouse model of inflammatory myopathy. Arthritis and Rheumatism, 64(11), 3750–3759. Publication

Alger, H. M., Raben, N., Pistilli, E., Francia, D., Rawat, R., Getnet, D., … Nagaraju, K. (2011). The role of tumor necrosis factor-α-related apoptosis-inducing ligand (TRAIL) in mediating autophagy in myositis skeletal muscle: A potential non-immune mechanism of muscle damage. Arthritis and Rheumatism, 63(11), 3448–3457. Publication

Coley, W., Rayavarapu, S., van der Meulen, J. H., Duba, A. S., & Nagaraju, K. (2013). Daily Supplementation of D-ribose Shows No Therapeutic Benefits in the MHC-I Transgenic Mouse Model of Inflammatory Myositis. PLoS ONE, 8(6), e65970. Publication

Li, C. K., Knopp, P., Moncrieffe, H., Singh, B., Shah, S., Nagaraju, K., … Wedderburn, L. R. (2009). Overexpression of MHC Class I Heavy Chain Protein in Young Skeletal Muscle Leads to Severe Myositis : Implications for Juvenile Myositis. The American Journal of Pathology, 175(3), 1030–1040. Publication